Plasmid

Part:BBa_J100375:Design

Designed by: Zachary Shaver   Group: Campbell M Lab   (2018-04-09)
Revision as of 20:34, 9 April 2018 by Zashaver (Talk | contribs)

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rClone Red with Riboswitch C Mutant #1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 658
    Illegal AgeI site found at 770
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The rClone Red plasmid was used instead of the tClone Red plasmid because RS-C, a translational riboswitch, is supposed to contain its own ribosome binding site (RBS) for it to function properly. rClone Red does not contain an RBS just upstream of the RFP sequence, so it allows for the proper testing of this RS-C mutant.


Source

This part is the rClone Red plasmid (J119384) with an inserted mutated RS-C sequence.

References