Regulatory
pCMV

Part:BBa_K2364000

Designed by: Asterios Arampatzis   Group: iGEM17_Greece   (2017-10-26)
Revision as of 22:06, 3 March 2018 by Ballhysaeugene (Talk | contribs)


pCMV 

  Human cytomegalovirus (CMV) immediate early enhancer and promoter. It is a generally strong mammalian

expression promoter that drives constitutive expression of genes under its control. However, the strength of the 

promoter varies significantly between different cell types; being very strong in some (e.g. 293T and CMMT) and
rather weak in others (e.g. MRC5 and MSC) (Qin Jane Yuxia, et al., 2010). 
  It has been shown in embryonic stem cells (ESCs) that the nuclear protein of 95kDa (Np95) interacts with regulatory domains of the de novo methyltransferases; Dnmt3a and Dnmt3b, and mediates silencing of the CMV promoter (Meilinger D. et al., 2009). Transcriptional silencing was also observed in rat muscles due to methylation of the CMV promoter enhancer (CMV-PE) 24h post-administration, showing decreased expression overtime (Brooks Alan R., et al., 2004) . Therefore, when using the CMV promoter, the type of cell as well as the time given to express the gene-of-interest should not be considered consistent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None