Part:BBa_I721003
Lead Binding Protein + Promoter (version b)
lead binding protein + promoter (part 8)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 479
Part BBa_1721003 is the Lead Binding Protein + Lead Promoter system
Description
This part is designed to enable Lead Detection in a cell. When Lead (Pb +2) enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I721003. BBa_I721003 contains both the Lead Binding Protein sequence and the Lead Promoter sequence, and should form a standalone Lead Detection system.
This sequence was obtained using PCR from the genome of Ralstonia metallidurans. Biobrick cut sites were added using specific primers during PCR.
This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "
This part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.
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