Part:BBa_K2262023

T7 Promoter+RBS+C-1.4

Figure 1. P_T7+RBS+C-1.4+terminator

Introduction

      C-1.4 is a peptide from Litoria xanthomera [1]. The score of SCM analysis shows its promising potential to be antifungal.

Scoring Card Predict

      C-1.4 got 401.2 from the Antifungal Scoring Card of Parabase System. The score is over 353, the threshold to divide whether a peptide has an antifungal function probability, that means C-1.4 has a high probability to have an antifungal function.

Figure 2. The sequence of C-1.4.
The colors of each amino acids represent the probability of anti-fungal dipeptides.

Experiment

1. Inhibition Zone

      The result shows that the place we put C-1.4 will have no hole. It means the peptide might not affect the growth of mycelium.

Figure 3. The result shows that C-1.4 has no function on affecting the growth of mycelium.

2. Spore Germination

      The result shows that the spore germination percentage of non-germinating spores adding C-1.4 with a concentration of 1000 μg/mL was higher than adding the negative control, which means the spore germination is inhibited by peptides and proves the peptides are effective. We can get the same result from adding C-1.4 with a concentration of 500 μg/mL and 250 μg/mL .

Figure 4. The result shows that C-1.4 may affect the germination of spore.

Reference

[1]SIMON T. STEINBORNER; GRAEME J. CURRIE; JOHN H. BOWIE; JOHN C. WALLACE; MICHAEL J. TYLER. "New antibiotic caerin 1 peptides from the skin secretion of the Australian tree frog Litoria chloris. Comparison of the activities of the caerin 1 peptides from the genus Litoria"Volume 51, Issue 2, February 1998, Pages 121–126.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]