Coding

Part:BBa_M50099:Design

Designed by: Daniel Tang   Group: Stanford BIOE44 - S11   (2017-10-19)
Revision as of 11:04, 13 December 2017 by Danieldt (Talk | contribs)

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Preproinsulin (w/ 6xHisTag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We modified this sequence to include a 6xHis Tag in the middle of the sequence, at residue 28 of the beta chain, immediately prior to an enzymatic cleavage site. His tagging allowed for subsequent measurement of preproinsulin/processed insulin using Western, ELISA, etc.


Source

Human preproinsulin

References

Sures, I., Goeddel, D.V., Gray, A., Ullrich, A. 1980. Nucleotide sequence of human preproinsulin complementary DNA. Science 208(4439) 57-9.