Part:BBa_M50096:Design
EL-222 - Light-sensitive transcription factor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 463
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 33
- 1000COMPATIBLE WITH RFC[1000]
EL-222 is a light-sensitive transcription factor that binds to DNA and drives transcription under blue light illumination. EL-222 consists of a LOV-HTH (helix-turn-helix) domain and VP16 activating unit. The LOV-HTH allows for light-gating of DNA binding; the LOV portion of the protein covers the HTH portion of the protein that binds DNA - under blue light exposure, the LOV domain changes conformation and reveals the HTH DNA binding site. The HTH binds to a sequence of c120 repeats. Once bound, the VP16 activating unit promotes transcription factors in the cell to drive genes downstream of the c120 repeats.
Design Notes
No changes were made to this sequence
Source
Bacterial transcription factor Supplemental Figures 2. "An optogenetic gene expression system with rapid activation and deactivation kinetics" Gardner et. al 2014, Nature Chemical Biology
References
"An optogenetic gene expression system with rapid activation and deactivation kinetics" Gardner et. al 2014, Nature Chemical Biology