Regulatory

Part:BBa_M50095:Design

Designed by: Daniel Tang   Group: Stanford BIOE44 - S11   (2017-10-19)
Revision as of 10:27, 13 December 2017 by Danieldt (Talk | contribs)


VP16 - Herpes simplex virus transactivating protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

VP16 or trans-activating tegument protein 16, is a viral protein sourced from human herpes simplex virus. VP16 promotes a number of human transcription factors and acts as an activator to drive downstream gene transcription.

Design Notes

No changes were made to this sequence as it is already optimized for HEK293T cells


Source

Human herpes simplex virus Supplemental Figures 2. "An optogenetic gene expression system with rapid activation and deactivation kinetics" Gardner et. al 2014, Nature Chemical Biology

References

"An optogenetic gene expression system with rapid activation and deactivation kinetics" Gardner et. al 2014, Nature Chemical Biology