Coding

Part:BBa_K2510113

Designed by: Aya Gomaa   Group: iGEM17_Paris_Bettencourt   (2017-10-27)
Revision as of 03:54, 2 November 2017 by Alma.chapet (Talk | contribs)


pdDronpa wt TetR

This part encodes for TetR in wtdronpa

In our project we developed a logic gate at the promoter level by creating dually repressed promoters using different combinations of the operators for TetR, P22 c2 and HK CI, the logic gate was tested with Dronpa-repressors fusions, the fluorescence levels increased over 1000-fold when violet light is shone




<img src="Logic_tetR_PB.png"> <img src="Logic_P22c2_PB.png"> <img src="Logic_HKcIfigure_PB.png"> 

                                           
                                           Figure 1:Results of the cell-free experiment. Each promoter was tested with its cognate repressors. Top: Testing with TetR caged with either wt-Dronpa (BBa_K2510108) or a mutated version(BBa_K2510109)Middle: Testing with P22c2 caged with either wt-Dronpa (BBa_K2510112) or a mutated version(BBa_K2510113).Bottom: Testing with HKcI caged with either wt-Dronpa (BBa_K2510110) or a mutated version(BBa_K2510111)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None