Regulatory

Part:BBa_K2379008

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-10-01)
Revision as of 03:30, 2 November 2017 by Azhagammal (Talk | contribs)

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Adaptor

This construct consists of the coding sequence of the tnaC leader peptide BBa_K2379000(BBa_K2379000) followed by the Rho-dependent terminator region BBa_K2379001(BBa_K2379001). It converts the translational regulatory property of a riboswitch to transcriptional regulation

Usage and Biology

The adaptor was characterised using the following three parts:

1) Adaptor with constitutive RBS:

T--SVCE_CHENNAI--result_graph_6.jpg

Further characterisation can be found in the link provided below:

BBa_K2379009(BBa_K2379009)

2) Adaptor with FourU RNA Thermometer:


T--SVCE_CHENNAI--result_graph_5.jpg

Further characterisation can be found in the link provided below:

BBa_K2379010(BBa_K2379010)

3) Adaptor with RNA pHMeter:

T--SVCE_CHENNAI--result_graph_3.jpg

Further characterisation can be found in the link provided below:

BBa_K2379011(BBa_K2379011)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None