Composite
Part:BBa_K2522002:Design
Designed by: Dallas McDonald Group: iGEM17_US_AFRL_CarrollHS (2017-10-03)
sfGFP expressed in the presence of AI-2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 141
Design Notes
This part is an improvement on the Part BBa_K117008. It functions to sense AI-2 still, but YFP is replaced with Super Folder Green Fluorescent Protein (sfGFP), a protein that glows much quicker (around 4 hours to become visible) and is more useful for a diagnostic approach. This plasmid was created from ligation using New England Biolabs products and protocols.
Source
Backbone acquired from parts kit and sfGFP insert from another lab