Composite

Part:BBa_K2260001:Design

Designed by: Rachelle Varga   Group: iGEM17_Calgary   (2017-10-25)
Revision as of 03:18, 2 November 2017 by Atika ibrahim (Talk | contribs)

phaC1J4 w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaC1J4 operon was codon optimized for use in E. coli using the codon optimization table. Lastly, histidine tags were added to the operon.

Source

The phaC1 was obtained from Pseudomonas aeruginosa, whereas phaJ4 was obtained from Pseudomonas putida.

References