Composite

Part:BBa_K2350014:Design

Designed by: CHEN-CHIA JIANG   Group: iGEM17_NYMU-Taipei   (2017-10-25)
Revision as of 03:14, 2 November 2017 by Chen-Chia (Talk | contribs) (→‎Design Notes)

Design Notes

PCR primers for backbone pPIGBACK (BBa_K2350009) to get Ampicillin resistance gene which has been processed a PstI site-directed mutagenesis
Forward: TGCGCTCTCGCTAGTGGTCAcgcggaacccctatttgttt
Reverse: CACCCGCTGCAAAGCACGATttaccaatgcttaatcagtg

PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase upstream
Forward: CAATATCCCGTAAAGTTTTT
Reverse: aaacaaataggggttccgcgTGACCACTAGCGAGAGCGCA

PCR primers for Synechoccocus elongatus PCC7942 genomic DNA to get Lycopene cyclase downstream
Forward: cactgattaagcattggtaaATCGTGCTTTGCAGCGGGTG
Reverse: CGGCGTTACCGTCACGTAGT


NOTE:
In order to process a 3 pieces fusion PCR, the PCR primers for Lycopene cyclase upstream and downstream are different from the primers showed in BBa_K2350007 and BBa_K2350008.


Source

Synechoccocus elongatus PCC7942 genomic DNA
Backbone pPIGBACK (BBa_K2350009)

References