Coding

Part:BBa_K2271013

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 03:10, 2 November 2017 by Pauline tot (Talk | contribs)

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Pex34p with copper inducible promotor

This part contains the coding sequence for the peroxin Pex34p with a copper inducible promoter ScGAL1 and the terminator ScADH1. The sequence is codon-optimized for S. cerevisiae.

Usage and Biology

Pex34p is a peroxisomal integral membrane protein that can act both, independently and in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 745
    Illegal BglII site found at 307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

Pex34 is assembled with a copper inducible promotor. Cultivating the cells in a medium with 0,5 uM copper conzentration leads to a medium strong induction of the promoter. Visible are more slightly enhanced peroxisomes than in the WT strain.

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Categories
Parameters
None