Reporter

Part:BBa_K2346006

Designed by: Li Jianan   Group: iGEM17_HFLS_H2Z_Hangzhou   (2017-10-23)
Revision as of 03:06, 2 November 2017 by Canallee (Talk | contribs)


Enhanced Sensor for Nitrite/Nitrate

This is a functional improved device for BBa_K381001(https://parts.igem.org/Part:BBa_K381001). We replaced the original reporter gene GFP with brighter reporter gene sfGFP, in the hope to improve the sensitivity for the device to detect nitrite and nitrate. The Pyear promoter will be switched on under the presence of nitrate or nitrite, translating P2 ogr. The P2 ogr will cause inducible PF promoter to produce even stronger downstream PoPS. We attached a RBS for convince of registry users.


BBa K2346003.png As we can see from the graph, there is significant difference between NiSAP’s response to nitrate(40mM) and Pyear+GFP’s response to nitrate(40mM). After 3 hours in the plate reader, NiSAP’s A.U. is nearly twice the magnitude of Pyear+GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 141


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