Coding

Part:BBa_K2194003

Designed by: Catherine Dunaway, Anna Guseva   Group: iGEM17_Rice   (2017-10-23)
Revision as of 02:59, 2 November 2017 by Ag103 (Talk | contribs)


cysPUWA Sulfate Transport System cds

The cysPUWA genes encode the sulfate ABC membrane transporter in Escherichia coli. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for cysP, cysU, cysW, and cysA in the context in which they appear in E. coli MG1655.

Biology and Usage

Cys P is a binding protein which increases the affinity for sulfate uptake; genes cysU, cysW encode the integral membrane proteins. cysA is an ATP-binding subunit.

Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI): lower growth rate for cysPUWA transformants in the presence of Cr(VI) may indicate the higher rate of chromium transport inside the cell.


Corrected c6, cys.png

Figure 1: Effect of Chromate on E. coli Growth


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2090
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 3828


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Parameters
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