Coding
TCepsE

Part:BBa_K2471014

Designed by: Armando Palacios Chaparro   Group: iGEM17_Tec-Chihuahua   (2017-10-27)
Revision as of 02:58, 2 November 2017 by Armando.Pach (Talk | contribs)

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glycosyltransferase family 2 protein [Bacillus subtilis]

This gene producesa protein that arrests flagellar rotation in a similar way to that of a clutch, this by disengaging motor force-generating elements in cells embedded in the biofilm matrix. The clutch is a simple, rapid, and potentially reversible form of motility control. This gene is sufficient to inhibit motility and does so by arresting flagellar rotation (Blair, K. M., 2008). Though the EPS operon is normally repressed in B. subtilis, it's beneficial for the original copy of the gene to be knocked out if its synthetically expressed. Although many bacterial flagellar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None