DNA

Part:BBa_K2460010

Designed by: Haiyun Liu   Group: iGEM17_Fudan_China   (2017-10-24)
Revision as of 02:53, 2 November 2017 by Liuhy (Talk | contribs)


φC31 attB

This part is improved from BBa_K1039035. This is a recombination site attB for phiC31 integrase (BBa_K1039012). When used with phiC31 attP (BBa_K2460006) and phiC31 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiC31 integrase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None