Part:BBa_K2255008
p3_E.coli (Rfc25)
This part is sequence coding for the D1 and D2 of the protein 3 of M13. Those domains are required for M13 adsorption and entry.
This part was design with Freiburg (Rfc25) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
The collection part use for the creation of phage-like particle, those part correspond to the domain D1 and D2 of the p3 protein. They allow the phage-like particle to target the specific bacterium. To test the specificity of our phage-like particle and to target other pathogenic bacterium we design a large scale of attachment protein. This part is the first one of M13 attachment protein collection.
All the parts of our collection :
| Pathogene | Filamentous phage | GI | Part ID |
|---|---|---|---|
| Escherichia coli | M13 (fd,ff)[1] | 927334 | BBa K2255008 |
| Neisseria gonorrheae | NgoΦ6[2] | 1260906 | BBa_K2255009 |
| Pseudomonas aeruginosa | Pf3[3] | 215374 | BBa_K2255010 |
| Ralstonia solanacearum | RSM1Φ[4] | 5179368 | BBa_K2255011 |
| RSS1Φ[4] | 4525385 | BBa_K2255012 | |
| Vibrio Cholerea | CTXΦ[5] | 26673076 | BBa_K2255013 |
| VFJΦ(fs2)[6] | 1261866 | BBa_K2255014 | |
| VGJΦ[7] | 1260523 | BBa_K2255015 | |
| Xanthomonas campestris | ΦLf[8] | 3730653 | BBa_K2255016 |
| Xylella fastidiosa | XfasM23[9] | 6203562 | BBa_K2255017 |
| Xanthomonas fucans | XacF1[10] | 17150318 | BBa_K2255018 |
Table showing the attachment proteins from various filamentous phages.
Usage and Biology
This biobrick will be use to engineer phage-like particles targetting E. coli, this will enable us to test the fonctionality of our particles. Check out our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page] to know more about their design.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
- ↑ Smeal, S. W., Schmitt, M. A., Pereira, R. R., Prasad, A. & Fisk, J. D. Simulation of the M13 life cycle I: Assembly of a genetically-structured deterministic chemical kinetic simulation. Virology 500, 259–274 (2017).
- ↑ Piekarowicz, A. et al. Neisseria gonorrhoeae Filamentous Phage NgoΦ6 Is Capable of Infecting a Variety of Gram-Negative Bacteria. J Virol 88, 1002–1010 (2014).
- ↑ Luiten, R. G., Schoenmakers, J. G. & Konings, R. N. The major coat protein gene of the filamentous Pseudomonas aeruginosa phage Pf3: absence of an N-terminal leader signal sequence. Nucleic Acids Res 11, 8073–8085 (1983).
- ↑ 4.0 4.1 T, K. et al. Genomic characterization of the filamentous integrative bacteriophages {phi}RSS1 and {phi}RSM1, which infect Ralstonia solanacearum., Genomic Characterization of the Filamentous Integrative Bacteriophages φRSS1 and φRSM1, Which Infect Ralstonia solanacearum. J Bacteriol 189, 189, 5792, 5792–5802 (2007).
- ↑ Heilpern, A. J. & Waldor, M. K. pIIICTX, a predicted CTXphi minor coat protein, can expand the host range of coliphage fd to include Vibrio cholerae. J. Bacteriol. 185, 1037–1044 (2003).
- ↑ Ikema, M. & Honma, Y. A novel filamentous phage, fs-2, of Vibrio cholerae O139. Microbiology 144, 1901–1906 (1998).
- ↑ Campos, J. et al. VGJφ, a Novel Filamentous Phage of Vibrio cholerae, Integrates into the Same Chromosomal Site as CTXφ. J. Bacteriol. 185, 5685–5696 (2003).
- ↑ Tseng, Y.-H., Lo, M.-C., Lin, K.-C., Pan, C.-C. & Chang, R.-Y. Characterization of filamentous bacteriophage ΦLf from Xanthomonas campestris pv. campestris. Journal of general virology 71, 1881–1884 (1990).
- ↑ Chen, J. & Civerolo, E. L. Morphological evidence for phages in Xylella fastidiosa. Virology Journal 5, 75 (2008).
- ↑ Ahmad, A. A., Askora, A., Kawasaki, T., Fujie, M. & Yamada, T. The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease. Front. Microbiol. 5, (2014).
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