DNA
Part:BBa_K2460003
Designed by: Haiyun Liu Group: iGEM17_Fudan_China (2017-10-21)
φBT1 attP
This is a recombination site attP for phiBT1 integrase (BBa_K2460001). When used with phiBT1 attB (BBa_K2460002) and phiBT1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiBT1 integrase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |