Composite

Part:BBa_K2379005

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-10-01)
Revision as of 02:27, 2 November 2017 by Azhagammal (Talk | contribs)


RNA thermometer (FourU) with GFP under the regulation of pLAC

The RNA thermometer (BBa_K115002) is under the regulation of pLAC promoter (BBa_R0011). Following the RNA thermometer, the construct contains GFP(BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). Expression of GFP is regulated by the RNA thermometer and hence will be produced only at 37 degree Celsius.

Usage and Biology

ABOUT THIS PART:

The part (BBa_K2379005) consists of RNA thermometer followed by GFP. The RNA thermometer(FourU) BBa_K115002(BBa_K115002) is a temperature sensitive element that shows higher expression of fluorescence at the temperature above 37ºC and lower expression below 37ºC. The part works as expected and maximum level of fluorescence was observed at 37ºC.

T--SVCE_CHENNAI--result_graph_4.jpg


CONCLUSION:

The expression of GFP was found to be basal and minimum at 32ºC. A sudden induction in the expression was observed at the temperature 37ºC and it seemed to be gradually decreasing after 42ºC. This behaviour of RNA thermometer(FourU) observed supports the data given by other teams. Thus, the RNA thermometer(FourU) works as expected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 765


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Parameters
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