Regulatory

Part:BBa_K2365041

Designed by: yuanqi jia   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 01:57, 2 November 2017 by Tinaqin (Talk | contribs)


TDH3 promoter

a Constitutive improved promoter from Saccharomyces cerevisiae

Glyceraldehyde-3-phosphate dehydrogenase, -3- three glyceraldehyde dehydrogenase promoter (tdh1, tdh2, tdh3, tdh1) for weak promoter, tdh3 promoter, 3 phosphoglyceraldehyde catalytic oxidation (dehydrogenation) and phosphorylation of 1,3- - generation, is the central link of glucose metabolism, plays an important role in sugar metabolism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


TDH3.jpg

Figure1:TDH3 from kit plate,the construct was transfected into Saccharomyces cerevisiae cells, the green fluorescence was observed under fluorescence microscope clearly. It indicates this part could work well.

Improved design:

We construct the device： TDH3 promoter+GFP+ADH1 terminator. TDH3 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence.

Characterization：

FIGURE .2.png

Figure2:The responses of promoter strengths of artificial TDH3 promoter and native promoter TDH3 were comprehensively compared. The strength of improved TDH3 was always higher than native promoter TDH3，but varied along with the genetic background of host.

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