Coding

Part:BBa_K2381001

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 01:57, 2 November 2017 by MinTT (Talk | contribs)


nMagHigh


nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms.
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts BBa_K2381019.
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403


[edit]
Categories
Parameters
None