Part:BBa_K2457000:Design

araC + pBAD arabinose inducible promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1207
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1042
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1024
  

Design Notes

E. coli

Source

E. coli

References

ENGLESBERG, E.; WILCOX G. Regulation: positive control. Annu. Rev. Genet. 8: 219–242. 1974.

GUZMAN, L. M.; BELIN, D.; CARSON M. J.; BECKWITH J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriology 177: 4121–4130. 1995.

KUHLMAN T. E. & COX E. C. Site-specific chromosomal integration of large synthetic constructs. Nucleic Acids Research. 38:1–10. doi: 10.1093/nar/gkp1193. 2010.

LEE N. L.; GIELOW W. O.; WALLACE R. G. Mechanism of araC autoregulation and the domains of two overlapping promoters, PC and PBAD, in the l-arabinose regulatory region of Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 78:752–756. 10.1073/pnas.78.2.752. 1981.

LEE N.; FRANCKLYN C.; HAMILTON E. P. Arabinose-induced binding of AraC protein to araI2 activates the araBAD operon promoter. Proc Natl Acad Sci U S A 84:8814–8818. 10.1073/pnas.84.24.8814. 1987.

SCHLEIF, R. AraC protein, regulation of the L-arabinose operon in Escherichia coli, and the light switch mechanism of AraC action. FEMS Microbiology Reviews, Volume 34, Issue 5, 1 September 2010, Pages 779–796. 2010.

SCHLEIF, R. "AraC Protein: A Love-Hate Relationship". BioEssays, Vol. 25, pg. 274-282. 2003.

YANOFSKY C.; PLATT T.; CRAWFORD I.P.; NICHOLS BP.; CHRISTIE GE.; HOROWITZ H.; VANCLEEMPUT M.; WU A.M. The complete nucleotide sequence of the tryptophan operon of Escherichia coli. Nucleic Acids Res. 1981;9:6647–68. 1981