Composite

Part:BBa_K2317005:Design

Designed by: Shan Wang   Group: iGEM17_Jilin_China   (2017-10-26)
Revision as of 01:41, 2 November 2017 by Eleven (Talk | contribs) (→‎Design Notes)


TA - DmpR - TfdB-JLU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 3116
    Illegal NgoMIV site found at 3779
    Illegal NgoMIV site found at 3805
    Illegal NgoMIV site found at 3890
    Illegal NgoMIV site found at 4430
    Illegal NgoMIV site found at 4621
    Illegal AgeI site found at 2572
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 310
    Illegal BsaI.rc site found at 851
    Illegal SapI.rc site found at 1508


Design Notes

This part is a conbination of several basic parts, and we systhesized it. We added a TAA stop codon in the front of the whole sequence to avoid unpredictable shift of coding frame.

Source

systhesis

References