Regulatory

Part:BBa_K2365033

Designed by: Tong Cheng   Group: iGEM17_NAU-CHINA   (2017-10-04)
Revision as of 00:49, 2 November 2017 by Tinaqin (Talk | contribs)

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PDC1 promotor

PDC1 Yeast promoter; Constitutive expression; come from Saccharomyces cerevisiae BY4742 genomic sequence;

pyruvate decarboxylase promoter, can be applied to the formation of CO2 pyruvate and acetaldehyde, relating to a stage of alcohol fermentation, exists in yeast and plants. Widely in a-ketoacid and aldehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 289


PCR result

Pdc1 pdc1-cyc1.jpeg
启动子全部.jpeg

Figure.The fluorescence of the four test devices and control transformed into Saccharomyces cerevisiae and inoculated in YPD broth was measured after 8h. Nevertheless, the attractiveness of yeast as a platform organism (especially for metabolic engineering applications) needs continued efforts to expand the set of tools available.

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