Regulatory

Part:BBa_K2361009:Experience

Designed by: Thijs Ettema   Group: iGEM17_Groningen   (2017-10-13)
Revision as of 00:39, 2 November 2017 by Matthijs Groningen (Talk | contribs) (→‎Applications of BBa_K2361009)


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Applications of BBa_K2361009

The part was validated by constructing an expression vector based on the native vector where the part was amplified from: pNZ8048. This native lactococcal plasmid was used as backbone to integrate the coding sequence for GFP behind the promoter. This enabled us to obtain data on its inducable character. This was done by inducing the cells with various amounts of nisin once the cells were in the exponential growth phase (OD600: 0.5-0.6). The graph below shows the expression of GFP over a time period of 6 hours. The values shown are a ratio of GFP emmission over the absorbance at 600 nm to correct for varying growthrates.


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UNIQf243a874b84678f9-partinfo-00000001-QINU UNIQf243a874b84678f9-partinfo-00000002-QINU