Regulatory

Part:BBa_K2194002

Designed by: Catherine Dunaway, Anna Guseva   Group: iGEM17_Rice   (2017-10-23)
Revision as of 00:23, 2 November 2017 by Catdunaway (Talk | contribs) (→‎Biology)


PgntK Negative Feedback Membrane Stress Promoter

The Pgntk promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntk promoter region. [1]


Biology and Usage

[1] Boyarskiy, Sergey et al. “Transcriptional Feedback Regulation of Efflux Protein Expression for Increased Tolerance to and Production of N-Butanol.” Metabolic Engineering 33 (2016): 130–137. Web. 28 Oct. 2017.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 216


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Categories
Parameters
None