Protein_Domain

Part:BBa_K2355033

Designed by: Cathrine Agnete Larsen   Group: iGEM17_DTU-Denmark   (2017-10-26)
Revision as of 00:21, 2 November 2017 by Chrysillispolhaus (Talk | contribs)

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Cleavable peptide sequence from Human Alpha-adducin

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is DDSDAATF.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

The peptides is cleaved by Bitis arietans and Bitis gabonica. It can be used to distinguish between these two Bitis snakes and Naja nigricollis.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for N21
Figure 1: Substrate with sequences DDSDAATF (well N21) incubated with the three different venoms.

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