Generator

Part:BBa_K2381019

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-23)
Revision as of 00:14, 2 November 2017 by Johnson Huang (Talk | contribs)


light-activated split Luciferase

the light-activated split Luciferase, used to verify the function of light-induced dimerization protein.

this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381013, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.

Fig.1 Prove the function of pMag & nMag. n=1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2595
    Illegal AgeI site found at 1442
    Illegal AgeI site found at 2734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2236


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Categories
Parameters
None