RNA

Part:BBa_K2381023

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-11-01)
Revision as of 00:01, 2 November 2017 by Johnson Huang (Talk | contribs)


PcpcG2-gRNA (light-control cell replication inhibitor) It’s a gRNA sequence containing a light-induced promoter, 20bp targeting at oriC and a full-length gRNA scaffold. It's function is blocking the replication initiation.

Its function is proved by OD measurement.

References
1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.
2. Schmidl, S. R., Sheth, R. U., Wu, A., & Tabor, J. J. (2014). Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol, 3(11), 820-831.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None