Composite

Part:BBa_K2440009

Designed by: Chushu Zhu   Group: iGEM17_NUDT_CHINA   (2017-10-27)
Revision as of 23:56, 1 November 2017 by Zhuchushu13 (Talk | contribs)


CMV promoter ->Flag tag -> hAgo2-> SV40 PA

This is the hAgo2 expressing plasmid.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 717
    Illegal NheI site found at 3425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1863
    Illegal XhoI site found at 2773
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3288
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3288
    Illegal NgoMIV site found at 726
    Illegal AgeI site found at 2561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1423


[edit]
Categories
Parameters
None