Coding

Part:BBa_K2317007:Design

Designed by: Shan Wang   Group: iGEM17_Jilin_China   (2017-10-27)
Revision as of 23:54, 1 November 2017 by Eleven (Talk | contribs) (→‎Design Notes)

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TfdB-JLU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 262
    Illegal NgoMIV site found at 925
    Illegal NgoMIV site found at 951
    Illegal NgoMIV site found at 1036
    Illegal NgoMIV site found at 1576
    Illegal NgoMIV site found at 1767
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TfdB-JLU gene has a high GC ratio of nuleotides, so it's not easy to get this fragment through normal PCR procedure. The forward PCR primer was easy to form hairpin itself and the sequence could not be avoided. We failed many times in getting this DNA fragment. Finally, we failed to to submit this part. But we synthesized it in our composite part.

Source

metagenomic screening

References