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Part:BBa_K2213005
PduD(1-20)_mCherry_cgPPK2_His6
The PduD(1-20) domain is sufficient to localise the part into a recombinant ethanolamine utilisation (Eut) or 1, 2 propanediol (Pdu) bacterial microcompartment (Jakobson et al., 2015). The mCherry allows monitoring the subcellular location of the part. The cgPPK2 domain encodes a soluble class II polyphosphate kinase from Corynebacterium glutamicim(Lindner et al., 2007).
References
Jakobson, C., Kim, E., Slininger, M., Chien, A. and Tullman-Ercek, D. (2015). Localization of Proteins to the 1,2-Propanediol Utilization Microcompartment by Non-native Signal Sequences Is Mediated by a Common Hydrophobic Motif. Journal of Biological Chemistry, 290(40), pp.24519-24533.
Lindner, S., Vidaurre, D., Willbold, S., Schoberth, S. and Wendisch, V. (2007). NCgl2620 Encodes a Class II Polyphosphate Kinase in Corynebacterium glutamicum. Applied and Environmental Microbiology, 73(15), pp.5026-5033.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 865
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 103
Illegal SapI.rc site found at 1544
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