Composite

Part:BBa_K2448035:Experience

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 23:21, 1 November 2017 by Brumake (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2448035

Screening protocol

E. coli cell were transformed either with this part or with it's Dpe mutated version. All the clones present on the plates after the transformation were cultivated and the psicose concentration was evaluated using the protocol described for BBa_K2448025 but in the presence of 50 g/L of fructose per well for 9 to 10 hours as this is the optimal measurement time according to our biosensor characterisation. All tests were performed in technical duplicates. Fluorescence measurements (mCherry) have been normalized on cell density (OD600).

Our Application

Screening of a library of mutants of our enzyme, the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum (BBa_K2448021).

User Reviews

UNIQ4076a635d5279386-partinfo-00000000-QINU UNIQ4076a635d5279386-partinfo-00000001-QINU