Regulatory

Part:BBa_K2365515

Designed by: yuanqi jia   Group: iGEM17_NAU-CHINA   (2017-10-23)
Revision as of 23:01, 1 November 2017 by Tinaqin (Talk | contribs)


Mutant of TEF1

Mutant of TEF1 Yeast promoter; Constitutive expression

Transcription elongation factor gene promoter of Saccharomyces cerevisiae constitutive expression promoter in the commonly used system. According to the element and registry and database of synthetic biology database bioparts for synthetic biology (http://npbiosys.scbit.org/regulatoryelementdetails/00007) design and synthesis of tef1 mutations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166


Tef mutant.jpeg

Measurements of specific fluorescence were performed using cells harvested from the logarithmic phase during growth in shake flasks.

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