Coding

Part:BBa_K2450251

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-10-21)
Revision as of 22:59, 1 November 2017 by Tinyref (Talk | contribs) (→‎Usage and Biology)

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TetR CFP

A Tet Repressor tagged with CFP. TetR binds to the tet operator to prevent the transcription of a downstream gene. Repression is relieved by tetracycline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

TetR functions as a homodimer to bind tet operator sequences and prevent the association of RNA polymerase with its promoter. Each TetR monomer has a DNA binding domain and a dimerisation domain. The DNA binding domain is a helix-turn-helix motif that associates with a tet operator sequence (TCCCTATCAGTGATAGAGA). Our complementary part, BBa_K2450301, contains 2 repeats of this sequence - one for each monomer.

CFP is a variation on Green Fluorescent Protein.

For more information on how this part was used in our project, please see our wiki: http://2017.igem.org/Team:Oxford

Functional Parameters

[edit]
Categories
Parameters
None