Coding

Part:BBa_K2271002:Design

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 22:58, 1 November 2017 by Pauline tot (Talk | contribs) (→‎References)

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Pex11-mVenus with ScPAB1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2132
    Illegal BglII site found at 701
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References

[1] Gurvitz A, et al. (2001) Saccharomyces cerevisiaer Adr1p governs fatty acid beta-oxidation and peroxisome proliferation by regulating POX1 and PEX11. J Biol. Chem 276(34):509-23 PMID: 7860627

[2] Zhou Y, et al. (2016). Harnessing Yeast Peroxisomes for Biosynthesis of Fatty-Acid-Derived Biofuels and Chemicals with Relived Side-Pathway Competition. J. Am. Chem. Soc., 138 (47), pp 15368–15377

[3] DeLoache W C, Russ Z N, et al. (2016). Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways. Nature communications 2016 Mar | 7:11152 | DOI: 10.1038/ncomms11152