Part:BBa_K2292000

Histidine kinase of competence regulon, comD

ComD is a part of a two components signal transduction system of the bacteria Streptococcus mutans. It is a kinase and membrane-associated receptor. After comD binded to the quorum sensing molecule CSP, the comD-CSP complex will phosphorylate comE. Then, the phosphorylated comE can turn on the transcription of downstream gene.

In our project, we replaced the downstream gene with GFP. Below is a picture showing how this part work in our construction

<img src="" style="display:block; margin:auto;">

Quorum sensing (QS), is a methodology of bacteria to communicate, just like language to us human. The bacteria learn their population situation by accepting or delivering quorum sensing molecules. Quorum sensing can work on bacteria of the same species and bacteria of different species as well. They can interact with the very specific molecule within same species just like native language; and interact with universal molecule inter-species, just like a universal foreign language.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]