Part:BBa_K2442202
Ptet + RBS + MtlR
This part contains the mtlR coding sequence along with the ptet promoter. mtlR coding sequence is derived from the mannitol operon from Pseudomonas Fluorescens. The ptet promoter is the well characterised part R0040. This Biobrick was used in conjunction with BBa_K2442203. By splitting the regulatory protein (mtlR) from the promoter it regulates (pmtle) allowed for greater control over the expression of GFP.
Usage and Biology
The mtlR regulatory protein is found naturally in Pseudomonas fluorescens. The mtlR protein has been described as a multi-sugar transcriptional regulator. There is some evidence to suggest that the protein functions as a dimer. Naturally this multi-sugar regulator functions to switch on sugar metabolism genes in P.Flourescens in the presence of organic sugars.
The aim of our project was to harness the natural regulatory abilities of the mtlR protein and use this alongside the promoter that it regulates (pmtle). We also aimed to combine this regulatory system with a reporter construct BBa_K2442203 to evoke GFP response. We used the sequence of the P.Flourescens mtlR gene to construct a coding sequence that was regulated upstream by the ptet promoter. By cotrasnforming E.coli cells with BBa_K2442202 and BBa_K2442203 we were able to create a sugar responsive assay that produced GFP via induction by sugars.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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