Part:BBa_K2384004

DsbA+MBP(Hg)

DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .

Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010. (BBa_K346003)The optimization of this sequence is more suitable for the expression of Bacillus megaterium.

Usage and Biology

Optimization Report

Gene Name:DSBA-MBP(Hg)

Expression System:Bacillus megaterium

Gene Length:963 (bp)

1.Codon Used Adjustment The best value is 1 for sequence optimization.

Figure 1:Before Codon Adjustment

Figure 2:After Codon Adjustment

2.Codon Used Distribution Show the relative codon used distribution

Figure 3:Before Optimization

Figure 4:After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Figure 5:Before Optimization

Figure 6:After Optimization

Figure 7:The expression of DsbA-MBP(Hg) detected using SDS-PAGE

Figure 8:Plasmid digested by PvuII

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 255
  Illegal BglII site found at 267
  Illegal BglII site found at 711
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]