Coding

Part:BBa_K2382012

Designed by: SHAO-CHI LO   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)
Revision as of 22:07, 1 November 2017 by Ptrlintw (Talk | contribs) (→‎Usage and Biology)

EAAAK


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

In our design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding. A rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK three times to maintain distance of two proteins and preventing from interrupting each other while folding.
The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently.


Characterization of the EAAAK

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021

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