Generator

Part:BBa_K145201:Experience

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-20)
Revision as of 21:54, 1 November 2017 by Slavil (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K145201

User Reviews

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BNathalie

The 2008 KUleuven team has tested this part. We added a GFP under TetR promoter to this part. Since TetR was constitutively produced, fluorescence was detected. This part works very good. More information can be found [http://2008.igem.org/Team:KULeuven/Data/Input here].

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User Reviews

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BNathalie

The iGEM Bulgaria 2017 team used this part. We added a GFP or RFP under Ptet promoter to this part. TetR is constitutively produced, but still some basal fluorescence was detected in an uninduced samples (left tube on the picture below, the right one is induced).

3shbtjA.jpg

After careful sequence analysis we discovered a LVA-like tag fused to TetR that is not mentioned into the part's description.

We generated a new part that is without the degradation tag and is in inverted orientation. You can have a look here: https://parts.igem.org/Part:BBa_K2515003

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