Composite

Part:BBa_K2457005

Designed by: Maria Astolfi, Giovanna Maklouf, Juliana Vitoriano   Group: iGEM17_Amazonas_Brazil   (2017-09-23)
Revision as of 21:42, 1 November 2017 by JulianaNV (Talk | contribs) (→‎Characterization)


Standardized pBAD_Cas9 device

descrever


Usage and Biology

Design

BBa K2457005 circuit.png

Figure 1: BBa K2457005 circuit

Characterization

CROUT 1.png

Sequencing

BBa K2457005 electropherogram.png

Figure 2: Sequencing electropherogram from BBa_K2457005.

BBa K2457005 aligmnent.png

Figure 3: Alignment of the designed sequence and our final construction from BBa_K2457005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1024


[edit]
Categories
Parameters
None