Composite

Part:BBa_K2378004

Designed by: Muhammad Farhan Maulana, Marvin Nathanael Iman, Zulhaerana Bahar   Group: iGEM17_ITB_Indonesia   (2017-10-27)
Revision as of 21:23, 1 November 2017 by Marvinnathanael (Talk | contribs)


pSal + PETase Coding Device

ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, https://parts.igem.org/Part:BBa_J61051) which is commonly activated by salicylate induction. Besides salicylate, NahR-pSal regulatory system is also known to have a certain degree of responsiveness in the presence of organic pollutants (OPs). This particular characteristic of NahR-pSal is utilized in iGEM 2017 Indonesia's project to detect the presence of plastic.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1303
    Illegal NotI site found at 2212
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1957
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None