Coding
Part:BBa_K2271003:Design
Designed by: Eric Behle Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
Pex11-Venus with galactose inducible promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 534
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
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References
[1] Gurvitz A, et al. (2001) Saccharomyces cerevisiaer Adr1p governs fatty acid beta-oxidation and peroxisome proliferation by regulating POX1 and PEX11. J Biol. Chem 276(34):509-23 PMID: 7860627
[2] Zhou Y, et al. (2016). Harnessing Yeast Peroxisomes for Biosynthesis of Fatty-Acid-Derived Biofuels and Chemicals with Relived Side-Pathway Competition. J. Am. Chem. Soc., 138 (47), pp 15368–15377