Coding

Part:BBa_K2378007

Designed by: Muhammad Farhan Maulana   Group: iGEM17_ITB_Indonesia   (2017-10-27)
Revision as of 20:46, 1 November 2017 by Farhan maulana (Talk | contribs)


IrrE Coding Sequence

This is improved coding sequence of IrrE from BBa_K729001 (https://parts.igem.org/Part:BBa_K729001). When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock.

Background of Improvement

We tried to analyse existing IrrE part from BBa_K729001 Team UCL London, and found some possible error in the coding sequence. The translated nucleotide is not a contiguous protein, which is shown by a stop codon in the middle of the coding sequence, continued by another start codon ~90 base downstream.

At first, we thought that it's the IrrE nature to have two separate active proteins, but after we find the Uniprot accession number Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 489
    Illegal AgeI site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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