Coding

Part:BBa_K2271003

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 20:45, 1 November 2017 by Pauline tot (Talk | contribs)


Pex11-Venus with galactose inducible promoter

This part contains the coding sequence for the peroxin Pex11p with the galactose inducible promoter ScGAL1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.

Usage and Biology

Pex11 is a peroxin located in the inner surface of the peroxisomal membrane and is required for peroxisome biogenesis [1]. Erdmann and Blobel have shown that the deletion of the PEX11 gene in S. cerevisiae results in cells with fewer, larger peroxisomes, whereas overexpression results in cells with a higher quantity of smaller peroxisomes [2].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None