Coding

Part:BBa_K2515001:Design

Designed by: Slavil Peykov   Group: iGEM17_Bulgaria   (2017-10-26)
Revision as of 20:38, 1 November 2017 by Slavil (Talk | contribs) (→‎Design Notes)

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mini Singlet Oxygen Generator (miniSOG)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has double TAA stop codon.

If you want to have this synthesized by IDT, order the following sequence: 

MiniSOG CDS
GAATTCGCGGCCGCTTCTAGATGGAAAAGAGCTTTGTGATTACCGACCCGCGTTT
GCCTGATAACCCGATTATTTTTGCCTCAGACGGATTTTTGGAACTGACCGAATAC
TCGCGCGAAGAGATTCTGGGTCGTAACGGTCGTTTCCTTCAGGGGCCGGAGACAG
ACCAAGCCACGGTTCAAAAGATCCGTGACGCAATCCGCGATCAACGCGAGATTAC
TGTTCAACTTATCAACTATACAAAATCTGGCAAAAAATTTTGGAACTTATTGCAC
TTGCAGCCGATGCGTGATCAAAAAGGTGAATTACAATACTTCATCGGAGTTCAGT
TGGATGGGTAATAATACTAGTAGCGGCCGCTGCAG

and then clone it in a pSB vector of your choice using the following primers:

C_BioBrick_Prefix-F: TATGAATTCGCGGCCGCTTCTAG

C_BioBrick_Suffix-R: TATCTGCAGCGGCCGCTACTAGTA

Note: C means cloning - these primers have 3 extra bases at their 5' ends so the EcoRI and PstI can cut efficiently. Do not use the regular BioBrick_Prefix-F and R pair instead - it won't work!

Source

This part was synthesized as gBlock by IDT. The coding sequence was obtained from the SnapGene software database.

References