DNA

Part:BBa_K1039035

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-16)
Revision as of 20:37, 1 November 2017 by Liuhy (Talk | contribs) (→‎Improvement and Characterization by Fudan_China 2017)

PhiC31 att B

The attB site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).

Reference: Amy C. Groth, Michele P. Calos, Phage Integrases: Biology and Applications, Journal of Molecular Biology, Volume 335, Issue 3, 16 January 2004, Pages 667-678, ISSN 0022-2836, http://dx.doi.org/10.1016/j.jmb.2003.09.082.

Improvement and Characterization by Fudan_China 2017

  • Group: Fudan_China 2017
  • Author: Haiyun Liu, Fudan University

We improved this part and characterized the kinetic characterization and the orthogonality between phiC31 and some other serine recombinase using this improved attB site (BBa_K24600010). For more information, go to Mycobacterium Phage Bxb1 gp35, DNA integrase


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None