![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2271141:Design
Designed by: Manuel Lentzen Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
Pex13 membrane anchor with mRuby
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 1980
Illegal BglII site found at 879
Illegal BglII site found at 916 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2335
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The membrane domain of Pex13 was desinged as a peroxisomal membrane anchor for proteins as previously described by Erdmann et al. (2004). We only used the amino acids 200-310 and added a triple GGGGS linker to allow an undisturbed c-terminal fusion of any protein of interest.
Source
UniProtKB - P80667 (PEX13_YEAST)
References
Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals - Erdmann et al., 2004